Technology

The Bach Impeller Solution

While many stem cell therapies are being developed in clinical trials, few have resolved how production of the therapies can be scaled up to meet the needs of the market or even to make the therapies commercially viable.

For stem cell production, stirred bioreactors with micro-carriers are recognized to produce better results than two dimensional structures, however stem cells are easily damaged as a result of fluid turbulence in stirred bioreactors. Also, the cells frequently are not uniformly distributed throughout the bioreactor vessels, resulting in differing growth conditions for the cells in multiple mixing zones, which significantly undermines the integrity of the growth process.

Many scale up challenges can be mitigated through known process optimization techniques, though the greatest challenge, that of propagating cells in ever increasing sizes of conventionally equipped bioreactors, has as a primary obstacle, the laws of physics. 

The Bach impeller operates in a fundamentally different manner than an axial flow impeller which is essentially a downward pumping device. Notably, with the Bach impeller the fluid does the mixing, not the impeller.

 

 

The Bach impeller draws fluid from the bottom of the vessel up into its conical structure and then expels the fluid to the sides of the structure, setting up a gentle uniform laminar flow of the liquid in the vessel, without damage associated with velocity differentials, hydrodynamic shear forces, differing mixing zones or uneven cell distribution.

Increasing the size of the Bach impellers to accommodate larger bioreactor vessels has absolutely no adverse effect on the process. Even the speed at which the Bach impeller operates can be increased to deal with larger volumes, without the high shear rates of axial flow impellers. The Bach impeller is fully scalable to large vessels, without the need to develop or adopt process optimization techniques to compensate for the laws of physics that plague conventionally equipped bioreactors. 

Even in small bioreactors, the cell propagation efficiency of the Bach impeller is considerably better than in conventionally equipped bioreactors using normal concentrations of micro-carriers and can be increased significantly due to the ability of the Bach impeller to hold higher concentrations of micro-carriers in suspension. This enhanced degree of efficiency per unit volume can be maintained even when scaling to larger vessels.

 

Limitations of Conventional Technology

Conventionally equipped bioreactors incorporate axial flow impellers to create the agitation required to hold stem cells in suspension during their growth phase. Importantly, with conventionally equipped bioreactors, the impeller does the mixing.  Though stem cells may survive agitation created by axial flow impellers in small bioreactor vessels, scaling up to larger vessels often requires the use of larger axial flow impellers to maintain cell suspension.

As the size of the axial flow impellers increase, the laws of physics dictate that speed at which the tip of the impellers move through the liquid, increases relative to the speed at which the hub of the impellers are turning. The velocity differentials along the axis of the impellers create hydrodynamic shear forces that can damage cells or even result in complete batch failure. For this reason, axial flow impellers are incompatible with effective scale up of stem cell production.